LOCUS       40924_24827       13468 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Targeting vector pHPRT8.9-Neo2(-) DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13468)
  AUTHORS   Saito S, Adachi N.
  TITLE     Gene-targeting assay at the HPRT locus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13468)
  AUTHORS   Adachi N, Saito S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2017) Contact:Shinta Saito Yokohama City 
            University, Graduate school of Nanobioscience; 22-2 Seto, 
            Kanazawa-ku, Yokohama, Kanagawa 236-0027, Japan
REFERENCE   3  (bases 1 to 13468)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13468)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-MAY-2017) Contact:Shinta Saito Yokohama City University, 
            Graduate school of Nanobioscience; 22-2 Seto, Kanazawa-ku, Yokohama,
            Kanagawa 236-0027, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2966..2977
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     regulatory      complement(4489..4780)
                     /label=PGK poly(A) signal
                     /note="PGK poly(A) signal"
                     /regulatory_class="polyA_signal_sequence"
     CDS             complement(4792..5592)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     promoter        complement(5606..6105)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     misc_feature    6115..11231
                     /gene="HPRT"
                     /label=3' arm
                     /note="3' arm"
     gene            6115..11231
                     /gene="HPRT"
                     /label=HPRT
     primer_bind     complement(11227..11243)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(11280..11298)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11319..11335)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11343..11359
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11367..11397)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11412..11433)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(11721..12309)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12483..13340)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(13341..13445)
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccat
      661 atcagtgtta ttaaatattt tgtatgccag gggctagaca taccaagaca accaatatgt
      721 ggttctactt aaataatatt agagtatctt ttatgatgac acttcatgag ttgactataa
      781 taatcttaga cttctaagag tttgggtttt caaaagatca cttagctttt ttgggtgatt
      841 tttccccctt actgtgagat gagagaggct gtttggattt gggattgggg tagcggggac
      901 agcaactttt cttttctttt tcttttttat tttgaggtag ggtattgctg tgtcacccag
      961 gctggagtgc agtggtgtga tctcggctca ctgcaacctc cacctcccgg gctcaggtga
     1021 tcctcctgct tcagcctccc cagtaactgg gactacaggc gcgtgccaca tgcctggcta
     1081 attttgtatt tttagtagag atggggtttc accatgttgg ccaggctggt ctctaactcc
     1141 tgacctcagg tgatacgccc acctgggcct cccaaaatac tgggattaca ggcatgagcc
     1201 gctgcatcag ccagcagttt ttcttgtggt tttttttgtt tgttttgttt tgttttgttt
     1261 ttgagatagg gtcttactct gttgtccacg ctggagtgct gtggtatgat cgtagctcac
     1321 tgcagcctca aactcctggg ctcaagtgat tccttctgcc tccgcctccc gagtagctgg
     1381 gactacaggt atgcaccacc atacctggca aatttttaca aagttttttg tagggacggg
     1441 gtcttgctac attccccatg tcggtcttga actcctggcc tcaagcaact ctcctgtctc
     1501 agcctcccaa agcactggga ttacaagtgt gagccaccac accatgccag tttttcctgt
     1561 tcagtgtgat attttatctt gttagactac agtgtgttaa aacttgtttt actaaatttt
     1621 caaacatact caaaagtgga gagaatagta taatgaatac ccgtatgttc atcacccatg
     1681 tttagaatat tattaaatat aaagattttg ctgcgtttgt cttagctctt taaaattttt
     1741 ctttttctct ttgtgaccta aaggaaattc catatcttat cactttactt ctacattctt
     1801 gactaagatg actaagacat atagttacat ggttttttgt tttgtttttt gttttttaaa
     1861 gacgaaatct cgctcttgtc ccccaggctg gagtgcaatg gtgccatctc agctcagtgc
     1921 aacctctgcc ttctgggtac aagcgattct cctgcctcag cctcccaagt agctgggatt
     1981 acaggctcct gccaccacgc ctggctaatt tttgtatttt tagtagagac ggcgggggga
     2041 ggtttcacca tgttgacaag gctggtctgg aactcctgac ctcaggtgat ccacccgcct
     2101 cggcctccca aagtgctggg attacaggcg tgagccaccg cgcccagcct gtttttttgt
     2161 ttgtttgttt tgtttttttt gagacagagt cttgctctgt ttcccaggct ggagtgaagt
     2221 ggcgcattct tggctcactg caaccttcac ctcccaggtt caagtgattc tcctgcctca
     2281 gcctcccaag tagctgggac tacaggcatg tgtcaccaca cccggctaat ttttttgtat
     2341 ttttagtaga gacgggattt caccgtgttg cccaggctgg tctcgaactc ctgagctcag
     2401 gcagtctgcc tgcctcagcc tcccaaagtg ctgggattac acgtgtgaac caacccgccc
     2461 ggcctgttgt tttcttacat aattcattat catacctaca aagttaacag ttactaatat
     2521 catcttacac ctaaatttct ctgatagact aaggttattt tttaacatct taatccaatc
     2581 aaatgtttgt atcctgtaat gctctcattg aaacagctat atttcttttt cagattagtg
     2641 atgatgaacc aggttatgac cttgatttat tttgcatacc taatcattat gctgaggatt
     2701 tggaaagggt gtttattcct catggactaa ttatggacag gtaagtaaga tcttaaaatg
     2761 aggtttttta ctttttcttg tgttaatttc aaacatcagc agctgttctg agtacttgct
     2821 atttgaacat aaactaggcc aacttattaa ataactgatg ctttctaaaa tcttctttat
     2881 taaaaataaa agaggagggc cttactaatt acttagtatc agttgtggta tagtgggact
     2941 ctgtagggac cagaacaaag taaacattga agggagatgg aagaaggaac tctagccaga
     3001 gtcttgcatt tctcagtcct aaacagggta atggactggg gctgaatcac atgaaggcaa
     3061 ggtcagattt ttattattat gcacatctag cttgaaaatt ttctgttaag tcaattacag
     3121 tgaaaaacct tacctggtat tgaatgcttg cattgtatgt ctggctattc tgtgttttta
     3181 ttttaaaatt ataatatcaa aatatttgtg ttataaaata ttctaactat ggaggccata
     3241 aacaagaaga ctaaagttct ctcctttcag ccttctgtac acatttcttc tcaagcactg
     3301 gctatgcatg tatactatat gcaaaagtac atatatacat ttatatttta acgtatgagt
     3361 atagttttaa atgttattgg acacttttaa tattagtgtg tctagagcta tctaatatat
     3421 tttaaaggtt gcatagcatt ctgtcttatg gagataccat aactgattta accagtccac
     3481 tattgataga cactattttg ttcttaccga ctgtactaga agaaacattc ttttacatgt
     3541 ttggtacttg ttcagcttta ttcaagtgga atttctgggt caaggggaaa gagtttattg
     3601 aatattttgg tattgccaaa ttttcctcta agaagttgaa tcattttata ctcctgatgt
     3661 tatatgagag tacctttctc ttcacaattt gtctcttttt tttttttttt tgagacaagg
     3721 tctctgttgc ccaggctggg gtgcagtgca gcagaatgat cacagttcac tgcagtctca
     3781 acctcctggg ttcaagcgat ccttccacct cagcctcctg agtagctggg actataggtg
     3841 tgcgccacca ctcccagcta atatttttat tttgtagaaa cagggttcgc catgttaccc
     3901 agcctcccaa agtgctggga ttacaggcat gagccactgg cccagtttct acagtctctc
     3961 ttaatattgt atattatcca agaaatttca tttaatcaga acctgccagt ctgataggtg
     4021 aaaatggtat cttgttttta tttgcattta aaaaaaatta tgatagtggt atgcttggtt
     4081 tttttgaagg tatcaaattt tttaccttat gaaacatgag ggcaaaggat gtgttacgtg
     4141 gaagatttaa aaaaaatttt taatgcattt ttttgagaca aggtcttgct ctattgtcca
     4201 ggctggagtg cagtggcaca atcacagttc actccagcct caacatcctg cactaaagtg
     4261 attttcccac ctcacctctc aagtagctgg gactacaggt acatgctacc atgcctggct
     4321 aatttttttt tttttgcagg catggggtct cactgtattg cccaggttgg tgtggaagtt
     4381 taatgactaa gaggtgtttg ttataaagtt taatgtatga aactttctat taaattcctg
     4441 attttatttc tgtaggactg aacgtcttgc tcgactctag aactagtcga tctatagatc
     4501 atgagtggga ggaatgagct ggcccttaat ttggttttgc ttgtttaaat tatgatatcc
     4561 aactatgaaa cattatcata aagcaatagt aaagagcctt cagtaaagag caggcattta
     4621 tctaatccca ccccaccccc acccccgtag ctccaatcct tccattcaaa atgtaggtac
     4681 tctgttctca cccttcttaa caaagtatga caggaaaaac ttccatttta gtggacatct
     4741 ttattgttta atagatcatc aatttctgca gacttacagc ggatcccctc agaagaactc
     4801 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     4861 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
     4921 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
     4981 gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcatc
     5041 gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     5101 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
     5161 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     5221 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     5281 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     5341 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     5401 ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     5461 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     5521 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     5581 ggccgatccc atattggctg caggtcgaaa ggcccggaga tgaggaagag gagaacagcg
     5641 cggcagacgt gcgcttttga agcgtgcaga atgccgggcc tccggaggac cttcgggcgc
     5701 ccgccccgcc cctgagcccg cccctgagcc cgcccccgga cccacccctt cccagcctct
     5761 gagcccagaa agcgaaggag caaagctgct attggccgct gccccaaagg cctacccgct
     5821 tccattgctc agcggtgctg tccatctgca cgagactagt gagacgtgct acttccattt
     5881 gtcacgtcct gcacgacgcg agctgcgggg cgggggggaa cttcctgact aggggaggag
     5941 tagaaggtgg cgcgaagggg ccaccaaaga acggagccgg ttggcgccta ccggtggatg
     6001 tggaatgtgt gcgaggccag aggccacttg tgtagcgcca agtgcccagc ggggctgcta
     6061 aagcgcatgc tccagactgc cttgggaaaa gcgcctcccc tacccggtag aattctcgag
     6121 atgtgatgaa ggagatggga ggccatcaca ttgtagccct ctgtgtgctc aaggggggct
     6181 ataaattctt tgctgacctg ctggattaca tcaaagcact gaatagaaat agtgatagat
     6241 ccattcctat gactgtagat tttatcagac tgaagagcta ttgtgtgagt atatttaata
     6301 tatgattctt tttagtggca acagtaggtt ttcttatatt ttctttgaat ctctgcaaac
     6361 catacttgct ttcatttcac ttggttacag tgagattttt ctaacatatt cactagtact
     6421 ttacatcaaa gccaatactg tttttttaaa actagtcacc ttggaggata tatacttatt
     6481 ttacaggtgt gtgtggtttt ttaaataaac tccttttagg aattgctgtt gggacttggg
     6541 atactttttt cactatacat actggtgaca gataccctct cttgagctac atcggtttgt
     6601 ggggagtcaa aagtcctttg gagctaggtt tgacaaataa ggtgggttaa cacttgtttc
     6661 ctagaaagca catggagagc tagagtattg gcgaattgaa gaaatccccc ttttttttta
     6721 acacacttaa gaaaggggac tgcaggtata ctcaagagag taagtcgcac cagaaaccac
     6781 ttttgatcca cagtctgcct gtgtcacaca attgaaatgc atcacaacat tgacactgtg
     6841 gatgaaacaa aatcagtgtg aattttagta gtgaatttca ttcataattt gatcgtgcaa
     6901 acgtttgatt tttattactt tagactattg tttctgattt tatgttgggt tggtatttcc
     6961 tgtgagttac tgttttcctt taaaatagga atttttcata ctcttcaaag attagaacaa
     7021 atgtccagtt tttgctgttt catgaatgag tcctgtccat ctttgtagaa actcgcctta
     7081 tgttcacatt tttattgaga ataagaccac ttatctacat ttaactatca acctcatcct
     7141 ctccattaat catctatttt agtgacccaa gtttttgacc ttttccatgt ttacatcaat
     7201 cctgtaggtg attgggcagc catttaagta ttattataga cattttcact atcccattaa
     7261 aaccctttat gcccatacat cataacacta cttcctaccc ataagctcct tttaacttgt
     7321 taaagtcttg cttgaattaa agacttgttt aaacacaaaa tttagacttt tactcaacaa
     7381 aagtgattga ttgattgatt gattgattga tggtttacag taggacttca ttctagtcat
     7441 tatagctgct ggcagtataa ctggccagcc tttaatacat tgctgcttag agtcaaagca
     7501 tgtacttaga gttggtatga tttatctttt tggtcttcta tagcctcctt ccccatcccc
     7561 atcagtctta atcagtcttg ttacgttatg actaatcttt ggggatttgt gcagaatgtt
     7621 attttagata agcaaaaacg agcaaaatag gggagtttaa ctttaatatt ttcttttaaa
     7681 aagcatttca tgttataaga tcaattctga gtggtagaaa tgctttgaca ttttatttcc
     7741 attttctact tttagttttt ttcctatttg tttaagatct tagaggatta ttaagctgaa
     7801 ctcctcaact gataaaaagc atgacatctt aaacataagc aaagcatatt tttaggttaa
     7861 ttttcacata gaaaacagtt tattttatgt gaaattctat gtagatatac tatttttttg
     7921 gtatttattg atatgtttat tttattttat tttattttat tttattttat tttattttat
     7981 ttatttattt ttttttttga gacagagtct cactctgttg cccaggctgg agtgcagtgg
     8041 catgatcgta gctcactgca acctccacct cccgggttca agcaattctt ctgtctcagc
     8101 ctcccgagta gctgggacta caggtgcctg ccactatgcc cggctaattt ttgtgttttt
     8161 agtagagatg gggtttcacc ttgttggtca ggctggtctc gaacccctga cctcaggtga
     8221 tccacccacc tcagcctccc aaagtgctgg gattataggc atgagccacc gtgcccggcc
     8281 gacatgttaa ttttttaaaa aaggctttac tggggtatat tttatataat ataataatca
     8341 catgttttaa ctatacaatt ccaagctttt tagtatattt atagggctat gcaaggaaga
     8401 tatactgtta aacagtagaa attgagaaag ctcttctgat aatatctctt gatttgatga
     8461 tggctcatgc ctgtaatctc agtgctttgg aaggccaaga cagcagaatc acttgaggcc
     8521 aggggttcga gaccagcctg ggcaacacag caatacccta tctttacaaa taataaaaat
     8581 atctgttgat ttgaagtaaa gttttttttt aaagacaagg tctcattctg tcacccaggc
     8641 tggaatgcag tagcaagatc acagctcact gtggccttga ccttctgggc tcaagtgatt
     8701 ctcccacttc ggcctcccga gtagctggga ctaacaggtg tgcaccacca tggctggcta
     8761 attttttttt atgtttgtag agattgggtc ttactgtgtt gcccaggctg atcccgaact
     8821 cctgggctca agcagtcttc ctgcctcagc ctctaaaatt gctgggatta caggcttgag
     8881 tcaccatgcc cagcctgaag tagcatttct accctgttta ataattcagc agcttgtcat
     8941 gtaagatatt catatatgca tataaaacat taggcagctt aatttggtaa aactgtaaaa
     9001 tggaaatttt aaattgtttg cagcatcaat aacattgatg tcagtatgat ttttacatgc
     9061 tgatcttgac caatttgaaa cagtgagtta aaatctggct gatccgtact aatcctaaag
     9121 aaatattcta tgaactatta aatgtttcca gaatatataa agaaacatta tgatgtcaac
     9181 acacccatct attttttttt ggaaataaaa actccatttt tcttattaaa gaaaacatgc
     9241 ttattagaaa acatacggct gggtgcagtg gcacacatgt aattccagtg ctttgggaga
     9301 tcgaggtggg agaatcactt gaggccagga gtttgagacc agcctagaca acataatgag
     9361 accccctctc tacacaaaaa gaattagttg tgcatggtgg cgtgcacctg tagtcccagc
     9421 tacttgggag gcagaggcag gagcatccct tgagcctagg agtttgagac tgcaggagtt
     9481 cgagactgag tggaatgcag tggaactgca ttccagcctg agtgacagag ggagaccctg
     9541 tcttaaaaaa ataagaaaga aaacacaact gcagaaaatt ataaaggatt taagtcattc
     9601 caaatatcac tgccactttt tatttagaat attctaaaga attctctctc tgtgtacaca
     9661 cacacatatg cgtactctta atccaagtag cttggtagga ttttatttac ctagtgccta
     9721 gatgggaaat tgcctgggga ttccaaatac ctatttcatt aaattaaaga tgtcactgat
     9781 tttaagactt aacactattt ttcatactgc caagaaagaa aacactacca gttataaatg
     9841 taaattgcca tcaattgtaa tacatcaatt ttagagctat tattaataaa atgtgaatgt
     9901 gcatcttaga gcaatgaaat atagtactat atatttgatg accttttctg ccctgtgata
     9961 ttcagaaagt gaaagttaaa tatgggctga gcatggtggc tcacacctgt aatcccagta
    10021 ctttgggaag tcaagacggg aggctggctt gaacccagga gttcaagacc agcctaggca
    10081 atgtagcgag acgccatctc aaaatattaa aaataagtaa ataagtaaat aaaaagaagg
    10141 ttaagtatac aaatgtattt cctttgttgt gaatttattt caattttata gtgatttttt
    10201 ttttttgaga cgaagtctca ctcttgtccc ccaggctgga gtgcgatggc gtgatctcag
    10261 ctcactgcaa cctctgcctc ccaggttcaa gctatactcc tgccttggcc ccccgagtag
    10321 ctgggattac aggcgcctgc taccatgcct ggctaatttt tgtattttta gttgagatgg
    10381 ggtttcacca tgttggccag gctggtctag aactcttgac ctctggtgat ccacccgcct
    10441 cggactccca aaatgctggg attacaggcg tgagccaccg tgcctggcca gtggtttttt
    10501 gttgttgttg ttgttgttgt tttgtttttg tttttgtttt tgttttgaga caggatcttg
    10561 ctctgtcacc caggctggag tgcagtggtg ccatcttggt tcactgcaac ctctgcgtgg
    10621 gctcaagcaa tcctcccacc tccctttcca gagtagcggg gaccacaggt gtgtgccacc
    10681 acacctgact aatttttgca tttttttttg tagaaacagg gttttgccat gttgcccagg
    10741 ttggtctgaa actcctgagc tcaaacaatc caactgcctt ggcttcccta agtgaaatta
    10801 caggcatggg ccactgtacc cagtctagtg atttttttat ttttattttt attttatttt
    10861 attttatttt tttaccaaaa aaacaacaaa gcctcaggag gaaaagttga tacacaagta
    10921 aattttattg gaaatgtttt tgtgtggacc ttaagcagag ggaaaattag tctgcattat
    10981 ggtgtatcca gactaaatga ctgatattaa aatgaaatta ttcttaggat ttgcaatctt
    11041 agagaaaact ttttcatttt tatttttttg agttacaaat tatcttcatt tacatttgag
    11101 aacagtgagt cacagaggga ttaagtaact tactcaagat catacaagtc tttgatttga
    11161 acccaatctt ttaactctgc agaactcaga gtcactctta tttggaaaaa ctttttaact
    11221 gatgtggatc cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc
    11281 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
    11341 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    11401 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    11461 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    11521 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    11581 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    11641 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    11701 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    11761 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    11821 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    11881 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    11941 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    12001 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    12061 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    12121 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
    12181 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    12241 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    12301 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    12361 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    12421 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    12481 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    12541 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    12601 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    12661 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    12721 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    12781 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    12841 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    12901 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    12961 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    13021 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    13081 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    13141 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    13201 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    13261 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    13321 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    13381 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    13441 ccgcgcacat ttccccgaaa agtgccac
//
